Medullary Thyroid Carcinoma Cell Lines – An Update

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Introduction: Medullary Thyroid Carcinoma (MTC) originates from calcitonin-producing neuroendocrine C-cells of the thyroid gland. Mutations in the RET-proto-oncogene are associated with both sporadic and familial MTC. As MTC are poorly responsive to chemo- and radiation- therapy, surgery is the only curative treatment at the moment. The cytogenetics of MTC have been sparsely investigated because the cells are very difficult to cultivate. In the last three decades our research group has established 10 continues cell lines derived from primary tumor and lymph node metastasis of patients with either sporadic or hereditary MTC.

Aim(s): In this study, we provide an overview of our MTC cell lines and compare them to the firstly established TT cell line.

Materials and methods: Immunocytochemistry and RT- PCR was used for the characterization of the cells. In vivo xenograft studies were performed to study the tumorigenicity of the cell lines.

Conference: 13th Annual ENETSConcerence (2016)

Presenting Author: Schwach G

Authors: Schwach G, Ghaffari-Tabrizi Wizsy N, Tam-Amersdorfer C, Meier-Allard N, Siddiqui Z,

Keywords: MTC continuous cell line, characterisation, xenografts,