Case Report: Whole Exon Sequencing of Primary Lesion and Metastatic Liver Lesion in Pancreatic Neuroendocrine Tumor


Introduction: Pancreatic neuroendocrine neoplasms(NENs) are classified into neuroendocrine tumors (NET) G1, G2, G3, and neuroendocrine carcinoma (NEC), which are different pathogenesis. The two kinds of tumors that occurs in the same lesion in one patient is very rare. We found high grade neuroendocrine neoplasms(NENG3) with a diameter of 0.1 cm in a patient with a pancreatic NETG2 (1.7 cm in diameter). The cells were significant atypia with Ki67 index of 60%, focal necrosis, while metastatic nodules in the liver (12, 0.1-1.1 cm in diameter) were all high grade NENG3.

Aim(s): To elucidate the difference in gene expression between primary lesion (NETG2) and liver metastases(NENG3), providing a reference for the pathogenesis of low-medium grade NET and high grade NENG3.

Materials and methods: A case of pancreatic NET with a significant heterogeneity between the primary and liver metastases (female, 52 years old) was selected. FFPE of the primary and metastatic lesion were subjected to whole exon sequencing (navasep, illumina). Bioinformatics methods were applied to analyze related pathways.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author:

Authors: Shi Y, Zhong D, Li Y, Niu Y, Tan H,

Keywords: pancreatic neuroendocrine neoplasms, whole exon sequencing, TRIOBP signaling pathway, MDM4,

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