Characterisation of new biomarkers from patients with neuroendocrine cancer using liquid biopsy methods

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Introduction: Neuroendocrine tumors originate from neuroendocrine cells. Clinicians diagnose and track these tumors not only but also by analysing patients' blood for biomarkers. Including liquid biopsy in early detection and monitoring can improve patient outcomes.

Aim(s): The study aims to develop a workflow for isolating circulating tumor cells, cell-free DNA, and genomic DNA, followed by genetic characterisation by CNVs, which will help identify patients' characteristics, allowing for the identification of tumor development and emerging resistance.

Materials and methods: We analysed cfDNA, gDNA, and CTC using a bioanalyzer, immunofluorescence, micro-manipulator, WGA method, and digital droplet PCR. We also performed CNV analysis and applied statistical methods to investigate this and clinical data.

Conference:

Presenting Author:

Authors: Pachnikova G, Jann H, Tacke F, Keilholz U,

Keywords: liquid biopsy, circulating tumor cells, cell-free DNA, neuroendocrine tumor, case study, copy number variations,