Characterising the tumor microenvironment of multifocal small intestinal NETs

#4166

Introduction: Small intestinal neuroendocrine tumors (SI-NETs) are thought to arise from enterochromaffin cells of the gut, often with multiple synchronous primary tumors. Recently, we showed that synchronous primary tumors from the same SI-NET patient display distinct somatic mutational profiles, suggesting that these tumors originate independently, despite few clear driver mutations. Thus, new mechanistic insights into multifocal SI-NETs are urgently needed.

Aim(s): To study the role of tumor microenvironment in the growth and development of multifocal SI-NETs.

Materials and methods: Our sample cohort included 75 synchronous primary tumors, 15 metastases, and their matched normal ileum from 13 patients with multifocal SI-NETs. We performed whole genome and single-nuclei RNA sequencing to study the tumor microbiome, stromal cell diversity, and gene expression in these lesions.

Conference:

Presenting Author:

Authors: Mäkinen N, Lee I, Dohlman A, Gao T, Zhang Z,

Keywords: small intestinal NET, tumor microenvironment, tumor microbiome,