Comparative Expression Analysis of Somatostatin Receptor Subtypes in Human Neuroendocrine Tumors Using Two Sets of New Monoclonal Antibodies

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Introduction: Expression of somatostatin receptor subtypes (sstr) is required for the successful use of somatostatin analogues (SSA) in the treatment of neuroendocrine tumors (NET). Reliable detection of sstr could improve the selection of SSA treatment. Rabbit and mouse monoclonal antibodies (mAbs) were recently developed independently in Jena and Basel.

Aim(s): To evaluate the utility of two sets of mAbs against sstr1, 2, 3, 5 in formaldehyde-fixed NETs.

Materials and methods: Newly generated mouse and rabbit mAbs specific to human sstr1, 2, 3, 5 were characterized with transfected HEK293 cells and normal pancreatic tissue. Comparative analysis of rabbit v. mouse mAbs was also conducted by immunostaining a tissue microarray (TMA) of 75 NET cores. Scoring was done with image analysis algorithms (Aperio).

Conference: 9th Annual ENETSConcerence (2012)

Presenting Author:

Authors: Lambertini C, Nuciforo P, Schulz S, Schmid H,

Keywords: sstr, NET, sstr-specific mAb,