Construction and comparison of non-functional pancreatic neuroendocrine tumor models

#3931

Introduction: Pancreatic neuroendocrine tumors (PNET) which account for more than 80% of pancreatic neuroendocrine neoplasm (pNEN) can be divided into functional and non-functional. The non-functional pancreatic neuroendocrine tumor (NF-pNET) without hormone-related clinical symptoms is frequently found by chance during physical examination or in large primaries and late stages of disease.

Aim(s): The mechanism of NF-pNET development is still in the exploratory stage, and for studying this relatively rare tumor, the experimental model is a key medium. The purpose of this study is to establish NF-pNET models and compare them from genotype to phenotype.

Materials and methods: The patient's tumor tissue collected from a NF-pNET patient with G3 grade was used to establish the cell line (named SPNE1), patient-derived xenograft(PDX), and patient-derived organoid (PDO). They were evaluated by immunohistochemical staining (IHC), whole-exome sequencing (WES) and single-cell transcriptome sequencing.

Conference:

Presenting Author: Wang Y

Authors: Wang Y, Ye Z, Lou X, Xu J, Zhou C,

Keywords: pancreatic neuroendocrine tumor, preclinical model, cell line, patient-derived organoid, patient-derived xenograft,