Decellularised pancreas explants as a novel 3D model for pancreatic neuroendocrine tumors (PNETs) biomarker discovery

#3793

Introduction: The lack of preclinical models of pancreatic neuroendocrine tumors (pNETs) challenges advancement in biomarker discovery. Culturing cancer cells in their native tissue enables the study of complex tumor cell-ECM interactions, products of which could be utilised as disease biomarkers.

Aim(s): Study the adaptation of the pNET cell proteome in response to culture in their native ECM. Study the value of tumor cell-ECM interaction products as disease biomarkers.

Materials and methods: Whole human pancreata were decellularised by perfusion and recellularised with BON-1 and QGP-1 cells. To study their adaptation to the native ECM, the proteomes of monolayer and 3D cultured cells was compared by LC-MS/MS. Enriched biological pathways and functional interactions were mapped using iPathwayGuide and STRING. The expression of top upregulated proteins was validated in surgically resected pNETs by IHC.

Conference:

Presenting Author: Ney A

Authors: Ney A, Al-Akkad W, Sedlak E, Garcia-Sampedro A, Hall A,

Keywords: Disease model, Tumor microenvironment, Biomarker discovery, Pancreatic NET,