Dissecting the role of collagen quantitation via tissue-tethered digital analysis in small bowel neuroendocrine tumours and related mesenteric tumour deposits

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Introduction: Small bowel neuroendocrine tumours (sb-NETs) and related mesenteric tumour deposits (MTDs) can present dense collagen and fibrosis. Despite well-known clinical consequences of this characteristic (bowel obstruction and ischemia), the relationship between SB-NETs and MTDs collagen deposition has been poorly evaluated.

Aim(s): We investigated the relationship between sb-NETs and MTDs fibrosis via tissue collagen quantitation (CQ).

Materials and methods: Sirius-Red-stained tissue sections of sb-NETs and MTDs were retrospectively collected for whole slide image (WSI) development with Leica Aperio AT2 scanner. WSIs were analysed for CQ percentage of tissue area occupied by collagen over whole tissue area of interest) via automated image analysis algorithm (Image-Scope, version 12.4). CQ was performed on sb-NETS and MTDs as a whole, and in sb-NETS mucosa/submucosa and serosa/subserosa.

Conference:

Presenting Author: Ali M

Authors: Ali M, Gambella A, Checchin F, Malerba D, Sambuceti V,

Keywords: neuroendocrine tumour, small intestine, collagen, fibrosis,