Dysregulated miRNA in patients with GEP-NEN and potential role as circulating biomarker

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Introduction: Neuroendocrine neoplasms (NENs) are a class of rare and molecularly extremely heterogeneous tumours. NENs arise predominantly in the gastrointestinal (GEP) and pulmonary tracts but can also involve thyroid and breast. NENs often present with non-specific symptoms and lack early specific biomarkers, leading to frequent metastatic diagnoses and primary site challenges.

Aim(s): This study aimed to identify common molecular and functional signatures across GEP- NENs and discover new diagnostic and prognostic biomarkers by employing a multi-omics approach, integrating multigene panel testing, transcriptome and miRNome profiling.

Materials and methods: MiRNome profiling (20 genes) was performed in a cohort of 66 patients with GEP-NENs of different grades. In order to assess the potential of circulating miRNAs in tumour progression, they were analysed in serum. Libraries were sequenced on the NextSeq 500 (Illumina) and processed using the iSmaRT pipeline.

Conference:

Presenting Author:

Authors: Di Mauro A, Clemente O, Cannella L, Della Vittoria G, Neri G,

Keywords: neuroendocrine tumour, miRNome profiling, biomarker, gep-net, molecular biology,