Effect of Combined Treatment with mTOR Inhibitors (mTORi) and Dopamine Agonists on Cell Proliferation in a Human Typical Lung Carcinoid Cell Line

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Introduction: The mTOR pathway and dopamine receptors are potential targets for treatment of neuroendocrine tumors (NET).

Aim(s): To evaluate the in vitro effect of combined treatment with the mTORi rapamycin (RAP) or everolimus (EVE) and the dopamine agonist cabergoline (CAB) on cell proliferation in a typical human lung carcinoid cell line (H727).

Materials and methods: The expression of some components of mTOR pathway and D2 receptor was assessed by RT-qPCR and immunostaining. The effect of mTORi and CAB on cell viability and cell cycle was evaluated by MTT assay and flow-cytometry, respectively.

Conference: 11th Annual ENETSConcerence (2014)

Presenting Author: Sarnataro M

Authors: Sarnataro M, Pivonello C, De Martino M, Sciammarella C, Cuomo G,

Keywords: mTOR, dopamine,

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