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Establishment and Characterization of Three GEP-NEC Cell Lines for In Vitro and In Vivo Studies


Introduction: To date chemotherapeutic concepts applying cisplatin/etoposide for the treatment of highly aggressive GEP-NEC do not provide convincing results. Thus, there is an urgent need for NEC cell line models that provide a reliable experimental system in identifying novel therapeutic targets.

Aim(s): Three NEC cell lines were established from a liver metastasis, a lymph node metastasis and an inguinal metastasis from patients with large cell NEC arising from the gastroesophageal junction, colon and anal canal, respectively.

Materials and methods: Neuroendocrine markers were analyzed by using RT-PCR and immunohistochemistry. Authenticity of the cell lines was verified by DNA fingerprinting and array CGH. In vivo tumorigenicity was evaluated in a mouse model and sensitivity against chemotherapeutic agents was assessed in vitro.

Conference: 13th Annual ENETSConcerence (2016)

Presenting Author: Krieg A

Authors: Krieg A, Riemer J, Dizdar L, Mersch S, Anlauf M,

Keywords: GEP-NEC,

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