Ex vivo expansion of TILs from panNET liver metastasis: In search of novel adoptive transfer strategies for the treatment of NETs

#4088

Introduction: It is unknown whether tumor infiltrating lymphocytes (TILs) from NETs may be an effective treatment in patients with NETs.

Aim(s): To investigate whether clinically relevant numbers of TILs may be expanded from panNET liver metastases and assess whether TILs may exert antitumor activity against NETs.

Materials and methods: Small fragments from panNET liver metastases were subjected to TIL isolation/expansion. TILs were expanded for up to 105 days and were phenotyped weekly to assess the lymphocyte composition, the % distribution of T cell differentiation subsets and the expression of exhaustion markers. The Seahorse technology was used to assess the metabolic status of TILs. Whole-exome sequencing and RNAseq data were generated and the neoantigen landscape of panNET liver metastasis is being assessed to identify neoantigen-reactive TILs.

Conference:

Presenting Author: Chaoul N

Authors: Chaoul N, d'Angelo G, Giglio A, Mandriani B, Matrella M,

Keywords: panNET, TILs, adoptive T cell therapy, neoantigen-reactive TILs,