Hepatic metastatic model establishment of pancreatic neuroendocrine tumor by hemi-splenectomy

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Introduction: The early diagnosis of pancreatic neuroendocrine tumor (pNET) patients is hard, and 60% patients are diagnosed with liver metastasis at the first visit to hospital. These patients are not suitable for surgery, which is the preferred and most effective option for pNETs. And liver metastasis promises worse outcome for patients.

Aim(s): Establishing hepatic metastases preclinical animal models is very vital and in desperate need to study the tumorigenesis, pathobiology, and the novel treatment for pNET.

Materials and methods: We developed a liver metastasis mouse model by hemi-splenectomy, and recorded IVIS (In Vivo Imaging system), PET/ct and anatomical image. Hemi-splenectomy includes: cell preparation, mouse preparation, laparotomy, tumor injection, abdominal closure and recovery from anesthesia, necropsy examination, harvesting liver.

Conference:

Presenting Author: Wang F

Authors: Wang F, Jing D, Zhou C, Qin Y, Chen J,

Keywords: Pancreatic neuroendocrine tumor, liver metastases, murine model, hemi-splenectomy, hepatic metastatic model,