Hypoxia down-regulates m6A methyltransferase METTL3 and WTAP promotes pancreatic neuroendocrine tumor metastasis via regulating TGFBI

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Introduction: Hypoxia is a marker of cancer progression. When faced with hypoxia, tumor cells exhibit the dynamic change of N6-methyladenine (m6A). m6A is a reversible RNA modification that plays an important role in gene expression regulation and is closely related to the occurrence and development of various tumors.

Aim(s): In order to adapt to the changes after hypoxia, cancer cells have more frequent cell communication with stromal cells. Therefore, it is increasingly significant to explore the correlation between cell communication mediators and hypoxia.

Materials and methods: Western blot and real time PCR was performed to detect gene expression. And we establish knockdown/overexpression cell model to explore METTL3 and WTAP function via CCK8, EdU, transwell and colony formation assay. MeRIP-seq and RNA-seq was executed to seek downstream genes. Subcutaneous tumor bearing model and hepatic metastasis model with caudal vein injection were used to detect the role of the molecules in vivo.

Conference:

Presenting Author: Ye M

Authors: Ye M, Bai J, Tian Y, Chen J, Lu F,

Keywords: Pancreatic neuroendocrine tumor, RNA methyltransferase, exosomes, tumor-associated macrophages, tumor metastasis,