Hypoxia upregulating ACSS2 enhances lipid metabolism reprogramming through HMGCS1 mediated PI3K/AKT/mTOR pathway to promote the progression of pancreatic neuroendocrine neoplasms

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Introduction: Pancreatic neuroendocrine neoplasms (pNENs) are relatively rare. Hypoxia and lipid metabolism-related gene acetyl-CoA synthetase 2 (ACSS2) is involved in tumor progression, but its role in pNENs is not revealed.

Aim(s): To investigate the role of hypoxia and lipid metabolism related gene ACSS2 in pNENs.

Materials and methods: mRNA and protein levels of ACSS2 and 3 hydroxy 3 methylglutaryl CoA synthase1 (HMGCS1) were detected using qRT-PCR and WB. The effects of ACSS2 and HMGCS1 on cell proliferation were examined using cell proliferation experiment,and their effects on cell migration and invasion were examined using transwell assay. The interaction between ACSS2 and HMGCS1 was verified by Co-IP experiments, and the functions of ACSS2 and HMGCS1 in vivo were determined by nude mouse xenografts.

Conference:

Presenting Author:

Authors: Gu D, Ye M, Zhu G, Bai J, Tang Q,

Keywords: hypoxia, ACSS2, lipid metabolism reprogramming, HMGCS1, PI3K/AKT/mTOR pathway, pancreatic neuroendocrine neoplasm,