Identification of new biomarkers associated with prognosis of pancreatic neuroendocrine neoplasms and establishment of survival prediction model

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Introduction: The Pan-NENs show high heterogeneity, and traditional classification cannot accurately predict tumor biological behavior and patient prognosis.

Aim(s): This study aims to identify the key genes closely related to the prognosis of patients with Pan-NENs, and reveal their effect via in vivo and in vitro experiments.

Materials and methods: Four Pan-NENs mRNA sequencing datasets and one microarray sequencing dataset were included. Differentially expressed genes (DEGs) between Pan-NENs tissues and normal tissues, G2 and G1grade tumors, G3 and G2 grade tumors were revealed, and then the overlapped DEGs were identified. Nomograms were established based on CEP55 and clinical characteristics. Short hairpin RNA(sh-RNA) targeting TPX2 or CEPP55 was respectively used to knock down TPX2 or CEP55 expression levels and the function of TPX2 and CEP55 was revealed in vivo and vitro experiments.

Conference:

Presenting Author: Yanling X

Authors: Yanling X, Pin Y, Mujie Y, Jianan B, Danyang G,

Keywords: pancreatic neuroendocrine neoplasm, biomarker, nomogram,