Immune heterogeneity and cascade of changes in subtypes of pancreatic neuroendocrine tumors

#3184

Introduction: Previously, we published four molecular subtypes of pancreatic neuroendocrine tumors (PanNETs) - met-like primary (MLP)-1 and -2, intermediate and insulinoma- like with different clinical and molecular characteristics. While immunotherapy trials are on-going in PanNETs, the immune landscape of this disease is largely unknown.

Aim(s): To systematically report the evolution of PanNETs associated with the cascade of changes in immune microenvironment and pathophysiology in molecular subtypes and study how this may impact upon immune cell type distribution, functioning and immune-based targeting.

Materials and methods: We analysed 207 patient fresh frozen PanNET samples from 3 different cohorts using different transcriptome profiling platforms. Subtype-specific immune gene expression, cell types, pathophysiology and pathways were analysed together using computational approaches. Multiplex immunohistochemistry was applied to validate the results.

Conference: 18th Annual ENETS Concerence (2021)

Presenting Author: Sadanandam A

Authors: Sadanandam A, Young K, Lawlor R, Ragulan C, Patil Y,

Keywords: pancreatic neuroendocrine tumor, immune microenvironment, viral-mimicry pathway, pancreatic cancer, computational methods, T cells, macrophages, immunotherapy, B cells, complement pathway, interferon signalling, STING pathway, hypoxia, necroptosis, tumor ,