Immunohistochemical (IHC) Overexpression of Succinate Dehydrogenase (SDHB) as Prognostic Factor in Sporadic Pheochromocytomas/Paragangliomas (P/P)
Introduction: Recently, gene mutations (SDH complex) involved in the pathogenesis of familial P/P have been discovered. These mutations are often associated with SDHB activity loss and activation of hypoxia signals.
Aim(s): SDHB IHC expression in sporadic P/P with limited or advanced disease.
Materials and methods: Eleven P/P treated from 1999 to 2009 were identified: median age 62 yrs (range 34-78), males/females = 4/7. 28% were in stage IV, with syncronous and metachronous mts in 33% and 37%, respectively. SDHB IHC expression were carried out in all primary tumor tissues using a semiquantitative scale, considering staining intensity (score 0-3). The staining for SDHB was optimized employing micro-slices of normal myocardial atrial muscle as positive control. So as to ensure antibody specificity, consecutive sections were incubated in the absence of primary antibody.
Conference: 9th Annual ENETSConcerence (2012)
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