m6A modifications promote the invasion and metastasis of pancreatic neuroendocrine neoplasms by activating the Integrin/FAK signalling pathway via TGFBI

#3987

Introduction: Nearly 50% of patients with pNENs have metastases at the time of initial diagnosis, and there is a lack of effective treatment once they have metastases. The 5-year overall survival rate for G3 pNENs with metastasis at diagnosis was 0, which was significantly lower than that for patients without metastasis, with a 5-year overall survival rate of 43%. At present, the pathogenesis of pNENs has not been clarified.

Aim(s): Therefore, exploring the molecular mechanism of pNENs' susceptibility to metastasis and potential drug therapeutic targets is of great clinical significance to improve the prognosis of pNENs patients.

Materials and methods: Through the establishment of knockdown/overexpression cell model, wound healing experiment, transwell, CCK8, clonal formation and other cell phenotypic experiments were performed to study genes function. Transcriptomics and RNA methylation sequencing were used to further search for downstream molecules and pathways. The effect of m6A modification on pNENs was studied by subcutaneous tumor formation and hepatic metastasis of tail vein in nude mice.

Conference:

Presenting Author:

Authors: Ye M, Tang Q,

Keywords: RNA methyltransferases, Tumor metastasis, Pancreatic neuroendocrine neoplasm, Transforming growth factor-β-induced gene, Integrin/FAK signaling pathway,