Metabolites in the tryptophan pathway as a diagnostic tool in neuroendocrine tumor patients

#3820

Introduction: A good and accessible biomarker is of great value in neuroendocrine tumor (NET) patients, especially considering its frequently indolent nature and long-term follow up.

Aim(s): We want to establish a sensitive and accurate method for the measurement of serotonin and other metabolites in the tryptophan pathway in plasma, and further demonstrate its utility as a biomarker in NET patients.

Materials and methods: We obtained plasma samples from 20 healthy controls and 149 NET patients followed at the University Hospital in Tromsø, Norway. Sampling was conducted carefully, using 9 mmol/l EDTA tubes, good blood flow emphasized and the first tube was discharged. Samples were centrifuged and stored at -80 until analysis. Samples were analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS).

Conference:

Presenting Author:

Authors: Johansen S, Hansen T, Meyer R, Jensen E, Goll R,

Keywords: serotonin, tryptophan pathway, LC-MS/MS, NEUROENDOCRINE TUMOR,