miR-1290 and miR-320c regulate cell proliferation and predict patient prognosis in neuroendocrine carcinomas

#4557

Introduction: The biology of neuroendocrine carcinomas (NECs) remains largely unknown, limiting the use of biomarkers for patient stratification and therapy response prediction in clinical practice.

Aim(s): This study aims to investigate the clinical relevance and functional role of miRNAs in NEC patients and cell models.

Materials and methods: We performed a pilot miRNome profiling by Next Generation Sequencing on serum samples from 18 NEC patients (pts) and 20 healthy donors (HD) enrolled in the SENECA trial (NCT03387592). The miRNAs that emerged as disease independent predictors were quantified through real-time PCR on a validation cohort (33 pts). Clinical variables, including treatments of enrolled pts, were collected and correlated with miRNAs expression levels. A functional analysis of the miRNAs effect on NEC cells was performed by transfecting BON-1 and QGP-1 with miR-mimics and inhibitors.

Conference:

Presenting Author: Liverani C

Authors: Liverani C, Sgolacchia F, Bongiovanni A, Calabrese C, Vanni S,

Keywords: biomarker, miRNAs, neuroendocrine carcinoma,