Optimising the establishment of patient-derived models for neuroendocrine neoplasms

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Introduction: Neuroendocrine neoplasms (NENs) are clinically and molecularly diverse, with limited understanding of their tumour biology. Efforts to develop patient-derived models have been unsuccessful, highlighting an urgent need for accurate models to support fundamental research.

Aim(s): To establish and characterise patient-derived models in vitro and in vivo.

Materials and methods: We collected 7 fresh tumour samples from untreated patients of different origins: six G1-2 GEP-NETs and one colon-NEC (CNEC). Small tumour fragments were used to establish: patient-derived xenografts (PDX), patient-derived organoids (PDTO) and PDX-derived organoids (PDXO). Growth factors were used to optimise organoid culture media. We assessed histology and neuroendocrine and proliferative markers by H&E and IHC staining for chromogranin A, synaptophysin and Ki-67.

Conference:

Presenting Author: Hernández Llorens M

Authors: Hernández-Llorens M, Baena-Moreno M, Lamas-Paz A, Sarmentero J, Anton-Pascual B,

Keywords: patient-derived model, organoids, PDXs, Growth factor,