Re-cellularised human pancreas 3D scaffolds as a novel disease model of pancreatic neuroendocrine tumors (pNETs)

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Introduction: A lack of preclinical models of pNETs challenges understanding of complex interactions between cancer cells and their stroma. Traditional in vitro disease models such as 2D cultures however, induce phenotypic and functional drifts consequent of their removal from the native, tissue specific niche. Culturing pNET cells in their native tissue recapitulates specific cell-ECM interactions and tissue remodeling processes.

Aim(s): To validate decellularized human pancreas derived ECM scaffolds as a more clinically relevant disease model, study the proteome adaptation of pNET cells cultured in their native environment and tissue specific interactions as drivers of disease progression.

Materials and methods: BON-1 and QGP-1 cells were cultured in decellularized pancreatic ECM scaffolds. Proteomic analysis (8-plex iTRAQ) was applied to study the proteome of cells grown in their native tissue compared to 2D cultures. Specific changes were validated in surgically resected PNET (G1-G3) by IHC.

Conference:

Presenting Author: Ney A

Authors: Ney A, Sedlak E, Garcia-Sampedro A, Hall A, Bhamra A,

Keywords: Disease model, pancreatic neuroendocrine tumor, tissue remodeling, stroma, biomarker,