Serum miRNA Expression in Small Intestine Neuroendocrine Tumors and Biological Function of miR-196a in Neuroendocrine Tumor Cells

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Introduction: MicroRNAs (miRNAs), post-transcriptional regulators, function either as tumor suppressors or oncogenes. We recently identified five upregulated and four downregulated miRNAs in small intestinal neuroendocrine tumor (SI-NET) tissue. We then started new analysis on body fluids to detect tumor signatures using patients’ serum.

Aim(s): To identify nine selected miRNAs expression from patients’ serum and explore the biological functions of potential miRNA target genes.

Materials and methods: Total RNA, keeping small RNAs, were isolated from 12 SI-NET serum samples (3 healthy controls, three primary tumors, three lymph node metastases and three liver metastases). QRT-PCR analysis studied miRNAs expression. Human NET cell lines were used as in vitro models to investigate miRNA targets, which were previously predicted by TargetScan analysis.

Conference: 10th Annual ENETSConcerence (2013)

Presenting Author:

Authors: Naboulsi R, Li S, Cui T, Öberg K, Giandomenico V,

Keywords: neuroendocrine tumors, serum miRNA expression, miRNA target functions,