Single-cell sequencing reveals the heterogeneity of pancreatic neuroendocrine tumors under the pattern of genomic instability and histological grading

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Introduction: Non-functional pancreatic neuroendocrine tumor (NF-Pan-NET) is a kind of highly heterogeneous tumors. Histological grading is the key factors affecting the prognosis of patients and instructive in guiding treatment and assessing recurrence in NF-Pan-NET. 90% of NF-Pan-NET are grade G1 or G2, leading to an unpredictable clinical course that varies from indolent to highly malignant. Approximately one-third of patients without CNV alteration and the prognosis of these patients are better than that of patients with CNV alteration. Therefore, probing the heterogeneity of tumors based on histological grading and CNV variation is crucial.

Aim(s): We aimed to explore the heterogeneity in NF-Pan-NET with different histological grading and genomic instability patterns, in addition to identifying the characteristics of patients with liver metastases.

Materials and methods: We recruited 17 NF-Pan-NET and analyzed their single-cell RNA-sequence (scRNA-seq), whole exome sequencing (WES), bulk RNA-sequencing, proteomics and phospho-proteomics to decode the malignant potential of NF-Pan-NET.

Conference:

Presenting Author: Ye Z

Authors: Ye Z, Zhou C, Xu X, Chen J, Yu X,

Keywords: Pancreatic neuroendocrine tumor, single-cell sequencing, genomic instability, histological grading,