Single-cell transcriptomic analysis of small intestinal neuroendocrine tumors revealed potential mechanisms of mesenteric fibrosis

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Introduction: Nearly half of patients with small intestinal neuroendocrine tumor (SI-NET) experienced local mesenteric fibrosis (MF), significantly impacting their prognosis. However, the mechanism by which SI-NET causes MF remains unclear.

Aim(s): We aim to elucidate the molecular mechanisms underlying SI-NET fibrosis and explore potential therapeutic targets.

Materials and methods: We conducted single-cell RNA sequencing (scRNA) on 5 primary tumor specimens and their corresponding adjacent non-tumor tissues, integrating one public SI-NET scRNA data. Samples were grouped into SI-NET with MF (SINET_MF) and without MF (SINET_NF) groups based on pathological or radiological data. We obtained bulk RNA-seq data from public databases and performed validation using immunohistochemistry staining and ELISA.

Conference:

Presenting Author:

Authors: Chen L, Lin X, Liu M, Li Y, Wen W,

Keywords: small intestinal neuroendocrine tumor, single-cell RNA sequencing, mesenteric fibrosis, fibroblasts,