Single-nucleus profiling of the tumour microenvironment in SI-NET patients

#4539

Introduction: Small intestinal neuroendocrine tumours (SI-NETs) are frequently present as multiple synchronous primary tumours in the small bowel. They are thought to arise from enterochromaffin (EC) cells in the gut, which account for less than 1% of the intestinal epithelium. Previously reported lack of shared somatic driver alterations in SI-NETs suggests that other, non-genetic mechanisms are likely involved in their tumorigenesis.

Aim(s): To study the role of tumour microenvironment in the growth and development of SI-NETs at single-cell level.

Materials and methods: Our sample cohort consisted of 11 primary ileal NETs, 6 metastases, and 23 normal ileal tissue samples from 13 multifocal and 10 unifocal SI-NET patients. Single-nucleus RNA sequencing (snRNA-seq) was performed using 10x Chromium Single Cell 5' High-Throughput v2 technology.

Conference:

Presenting Author: Mäkinen N

Authors: Mäkinen N, Kasai Y, Kim G, Thirlwell C, Nakakura E,

Keywords: small intestinal NET, tumour microenvironment, single-nucleus sequencing,