Thymosin Beta 4 Is an Autocrine Mitogen for Neuroendocrine Tumour Cells


Introduction: The most commonly used NET marker chromogranin A, has a sensitivity between 60-90% and a specificity of less than 50%, hence better NET markers are needed. Previous secretome analysis using BON1, NCI-H727 and SHP-77 cell lines has led to the discovery of various candidate NET markers. One such marker is Thymosin Beta 4 (TB4). TB4 is a small 43 amino acid intracellular peptide first isolated from bovine thymus tissue.TB4 or its oxidised form TB4SO are thought to play a role in various aspects of cancer biology including tumour invasion, metastasis and apoptosis.

Aim(s): To test using the human NET cell lines BON1 and QGP1 whether TB4 is an autocrine mitogen of NETs and plays a role in ERK signalling.

Materials and methods: BON1 and QGP1 cell cultures were serum starved and incubated with different concentrations of TB4, TB4SO and ERK inhibitor for different times to explore changes in ERK signalling and cell proliferation.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author:

Authors: Balasundaram P, Beaumont N, Liang S, Cuenco J, Lu X,

Keywords: neuroendocrine tumour, biomarkers, signalling,

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