Transcriptomic analysis of PanNET tumor progression from microtumor to metastasis in MEN1 patients

#4158

Introduction: Pancreatic Neuroendocrine Neoplasms (PanNENs) are a major component the Multiple Endocrine Neoplasia 1 Syndrome (MEN1). Although multiple pancreatic neuroendocrine microtumors (microadenomatosis) are a histomorphological hallmark of MEN1, their molecular status compared to PanNET as well as their metastasis remains unclear.

Aim(s): In this study we aim to understand the mechanisms underlying PanNET progression in MEN1 patients by comparing transcription profiles of pancreatic microtumors to tumor and their metastases of the same patient.

Materials and methods: Five MEN1 patients with resected pancreatic lesions in different stages (normal islets, microtumors, PanNET and metastases) were included. Individual lesions have been dissected using laser microdissection from PAXgene fixed material. RNAseq analysis of pooled normal pancreatic islets, 8 microadenomas, 4 PanNET and 1 PanNET metastasis was performed.

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Authors: Chouchane A, Kirchner P, Sylvain Maire R, Schiavo Lena M, Falconi M,

Keywords: Microtumor, MEN1, PanNETs, RNAseq, Transcriptomics, Progression,