Whole Exome Sequence Of BON-1 And QGP-1, Two Human Pancreatic Neuroendocrine Tumor Cell Lines, Reveals Homozygous Loss Of Function Mutations In TP53, HRNR and CYFIP2.


Introduction: The human BON-1 and QGP-1 cell lines are two models often used in pancreatic neuroendocrine tumor (PNET) research. Data on protein-changing mutations in these cell lines is lacking.


Materials and methods: Using the HiSeq 1000 next-generation DNA sequencing platform (Illumina), we sequenced the exome of the QGP-1 and BON-1 cell lines. Exonic sequences were enriched using the Truseq Exome Enrichment Kit (Illumina). Paired-end reads were aligned to the human reference genome. Single Nucleotide Variants (SNVs) and indels were called using the Genome Analysis ToolKit.

Conference: 10th Annual ENETSConcerence (2013)

Presenting Author: Vandamme T

Authors: Vandamme T, Op de Beeck K, Vangestel C, Pauwels P, Van Camp G,

Keywords: ,

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