Aberrant Tryptophan Metabolism in Stromal Cells Is Associated with Mesenteric Fibrosis in Small Intestinal Neuroendocrine Tumours


Introduction: Increased levels of serotonin secretion are associated with mesenteric fibrosis (MF) in small intestinal neuroendocrine tumors (SI-NETs). However, only a proportion of patients with increased serotonin production will develop mesenteric fibrosis.

Aim(s): Our aim was to gain insight in the variable profibrotic potential of serotonin by analyzing the tryptophan metabolism in primary SI-NETs and the associated mesenteric mass.

Materials and methods: The tumor cell and stromal compartments from the primary tumor and mesenteric mass of SI-NET patients with MF (n=6) and without MF (n=6) were analyzed by label-free proteomics. The KEGG tryptophan metabolism pathway was used to select identified proteins. Differential expression of selected proteins between patients with MF and without MF was analyzed using spectral index (SpI) analysis. Differential expressed proteins were validated by immunohistochemistry.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author: Blazevic A

Authors: Blazevic A, Iyer A, Van Velthuysen M, Hofland J, Franssen G,

Keywords: Small-intestinal neuroendocrine tumors, mesenteric fibrosis, serotonin, tryptophan metabolism, proteomics,

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