ANXA1-labelled cancer-associated fibroblasts induces tumour invasion and immunosuppressive microenvironment

#4519

Introduction: The growth pattern of a tumour is a marker for distinguishing inert from invasive tumours. In general, tumours such as the encapsulated follicular variant of papillary thyroid carcinoma show expansive growth. In contrast, invasive tumours, such as colorectal cancer and squamous cell carcinoma of the tongue, show invasive growth with infiltration into surrounding structures and have a poorer prognosis. Pancreatic neuroendocrine neoplasm (PNEN) is the heterogeneous tumour originating from peptidergic neurons and neuroendocrine cells. PNEN with a high proportion of tumour mesenchyme, especially those presenting an infiltrating stroma, have a poor prognosis. In the infiltrating mesenchyme, cancer-associated fibroblasts (CAF) play an important role. However, little research has been done on the mechanisms of how infiltrating CAF affects tumour malignancy and immune microenvironment.

Aim(s): The present study sought to elucidate the mechanisms by which invasive CAF induces increased malignancy and an immunosuppressive microenvironment in pNEN.

Materials and methods: Targeting specific CAF subpopulations in infiltrating tumours by single-cell RNA analysis. Primary CAF were extracted and co-cultured with BON1 and QGP1 cell lines to explore the mechanism of interaction.

Conference:

Presenting Author: Wang Y

Authors: Wang Y, Xu J, Chen J, Xu X, Ji S,

Keywords: pancreatic neuroendocrine neoplasm, tumour-associated fibroblasts, immunosuppressive microenvironment,