Cannabinoids as Inhibitor of Tumour Growth and Invasiveness in Small Intestine Neuroendocrine Cancer

#1560

Introduction: Tumour cell invasion and proliferation is a key factor for the prognosis and progression of cancer. A recent study revealed that cannabinoids can inhibit tumour invasion and proliferation in breast cancer through the down-regulation of ID-1, an inhibitor of helix-turn-helix transcription factors.

Aim(s): ID1 is highly expressed in the small intestine neuroendocrine tumour cell line P-STS. We therefore hypothesize that cannabinoids can also inhibit proliferation and invasion of P-STS via the down-regulation of ID-1.

Materials and methods: P-STS cells were treated with the CB-receptor agonist WIN-55,212. Subsequently, the ID1 expression was observed and proliferation, viability, apoptosis and mitosis were assessed. The tumour cells were xenografted onto a chicken chorioallantoic membrane (CAM) and the tissue was further stained immunohistochemically for Ki67 and a TUNEL-Assay was performed. Angiogenesis was assessed with CAM-Assays.

Conference: 13th Annual ENETSConcerence (2016)

Presenting Author: Errampalli R

Authors: Errampalli R, Reicher A, Kleinegger F, Hirschnöck T, Pfragner R,

Keywords: si-net, id1, cannabinoid​,