Comprehensive genetic analysis of tumor suppressor genes Men1, PTEN, Rb1 and p53 in NETs in mice

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Introduction: Tumor suppressor genes (TSGs) RB1, TP53 and PTEN are three of the most commonly mutated TSGs in human cancers. Multiple endocrine neoplasia type 1 (MEN1) gene is one of the most frequently mutated TSGs in human neuroendocrine tumors (NETs). Cooperative effects of genetic alterations of TSGs are frequently observed during carcinogenesis.

Aim(s): To better understand the molecular mechanisms of these TSGs/pathways on NETs, we investigated whether these four TSGs have cooperative effects mutually in suppressing NETs in mice.

Materials and methods: We generated double homozygous deletion of these four TSGs in pairs in transgenic insulin 2 gene expressing cells using the Cre-LoxP system. We also generated double homozygous deletion of Men1 and Pten in the knock-in mouse insulin 1 gene expressing cells using Cre-LoxP system. Systematic mouse genetics and histologic analysis were performed on these mice.

Conference: 18th Annual ENETS Concerence (2021)

Presenting Author:

Authors: Xu E, Vosburgh E, Wong C, Tang L, Levine A,

Keywords: neuroendocrine tumor, men1, rb, pten, p53,