Development of new 3D in vitro models for pancreatic neuroendocrine tumours (PanNETs)

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Introduction: Current PanNET in vitro models struggle to accurately replicate the tumour’s biology and microenvironment, limiting understanding of the mechanisms underlying PanNET behaviour and drugs response.

Aim(s): To develop: 1) 3D bioprinted PanNET models that replicate tumour-stroma interactions and support studies on tumour biology 2) patient-derived PanNET slices for personalised drug testing.

Materials and methods: 1) 3D bioprinted scaffolds were designed using PanNET cell lines (BON1, NT3, NT18P, NT18LM), human pancreatic stellate cells, and endothelial cells (HUVEC) within a hydrogel-based bioink. Both simple (single-cell type) and complex (co-culture) models were created. 2) PanNET slices, 350μm in thickness, derived from surgical specimens were generated by vibratome cutting. PanNET slices response to Everolimus, Sunitinib, and Temozolomide was evaluated.

Conference:

Presenting Author:

Authors: Battistella A, Pinos R, Barbaglio F, Capurso G, Schiavo Lena M,

Keywords: Preclinical models, Pan NET, microenvironment, bioprinting, tumour slices, drug testing,