Establishment of neuroendocrine neoplasms organoid biobank enables genotype-phenotype mapping

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Introduction: Gastroentero-pancreatic (GEP) neuroendocrine neoplasm (NEN) that consists of neuroendocrine tumor (NET) and neuroendocrine carcinoma (NEC) is a lethal but under-investigated disease owing to its rarity. Incidence of this disease is recently increasing, and novel treatment is warranted. However, the establishment and application of GEP-NEN disease models have been limited.

Aim(s): To establish an organoid biobank platform for GEP-NEN and to perform a comprehensive analysis.

Materials and methods: We established a GEP-NEN organoid biobank and performed a comprehensive analysis including whole-genome sequencing, RNA-seq and ATAC-seq. We also performed genetic engineering and reconstituted GEP-NEC from normal colon organoids.

Conference: 18th Annual ENETS Concerence (2021)

Presenting Author:

Authors: Kawasaki K, Fujii M, Kudo A, Kanai T, Nakagawa H,

Keywords: organoids, CRISPR-Cas9, multi-omics analysis, gastrinoma, MiNEN,