Evaluation of tumor tissue miRNAs for assessing the grade of pancreatic neuroendocrine tumors from FFPE samples

#3780

Introduction: Our research aims were to determine whether the human tissue microRNAs miR-21, miR-96, miR-130b and miR-210 are of use in the differential diagnosis of grade 1 and grade 2 pancreatic neuroendocrine tumors (PNET).

Aim(s): The microRNAs examined are implicated in the biology of PNETs based on the literature and could be potentially used as diagnostic markers in FFPE (formalin-fixed paraffin embedded) tumor tissues and eventually in liquid biopsy samples, too.

Materials and methods: In total, we collected 25 pancreatic neuroendocrine tumors from FFPE archived patient samples (16 grade 1 and 9 grade 2). These samples were sectioned for RNA isolation and sections were evaluated by expert pathologists to have the grade and region of interest containing the tumor tissue only. Total RNA was isolated with Recover ALL Kit (Thermo Fisher Scientific) from 80 µm of FFPE sections and the expression levels of miR-21, miR-96, miR-130b and miR-210 were determined and analysed compared to the geometric mean of RNU48 internal control and cel-miR-39 external spike-in-control as calibrators by quantitative RT-qPCR on a Taqman platform.

Conference:

Presenting Author: Nyirő G

Authors: Nyirő G, Szeredás B, Moloney C, Turai P, Vékony B,

Keywords: PNET, FFPE, RT-QPCR, miR-21, miR-96, miR-130b, miR-210, miRNA, Grade 1, Grade 2,