Everolimus Reduces Cell Viability in Selected Human Medullary Thyroid Carcinoma Primary Cultures

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Introduction: It has been demonstrated that everolimus, an mTOR inhibitor, has a potent anti-proliferative effect in a human Medullary Thyroid Carcinoma (MTC) cell line, TT, and in two human MTC primary cultures.

Aim(s): To evaluate the possible antiproliferative effects of RAD001 in a group of 20 human MTC in a primary culture.

Materials and methods: Twenty MTCs were dispersed in primary cultures, treated without or with 1 nM - 1 micro M RAD001, 10 nM SOM230, and/or 50 nM IGF-I. Cell viability and apoptosis were evaluated after 48 h and Calcitonin (CT) secretion was assessed after an 8 h incubation. Somatostatin receptor expression was investigated by quantitative PCR.

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Minoia M, Filieri C, Tagliati F, Buratto M, Ambrosio M,

Keywords: medullary thyroid cancer, everolimus, mTOR, antiproliferative effects,