Exploring the splicing landscape to identify survival-related biomarkers in small intestine neuroendocrine neoplasms

#4587

Introduction: The study of small intestine neuroendocrine neoplasms (siNENs) is challenging due to their rarity and complexity. While transcriptomic subtypes have been identified, the mechanisms behind their progression are still unclear. The process of RNA splicing is often altered in cancer, and our group has described that such dysregulation is also present in various NENs.

Aim(s): The aim is to analyse the splicing landscape in 43 siNENs, to find new splicing-related biomarkers associated with clinical features, which could enhance our understanding of siNEN biology.

Materials and methods: We analysed the clinical data and the transcriptome of a set of paraffin-embedded samples, comprising 26 long- (>5) and 17 short-term (<5 years) survivors. Gene expression (htseq-count) and differentially expressed genes (DESeq2) were quantified, along with specific splicing events (rMATS). Additional analyses were conducted with various R packages, Metaboanalyst, and SpliceTools software.

Conference:

Presenting Author: Ibáñez Costa A

Authors: Ibáñez-Costa A, García Vioque V, Pedraza-Arévalo S, Hernando Cubero J, García A,

Keywords: small intestine neuroendocrine tumour, splicing, biomarker, survival, ki-67, RNA,