Expression and Role of the CXCR4/CXCL12/CXCR7 Axis and Crosstalk with the mTOR Pathway in Neuroendocrine Tumors (NETs)

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Introduction: Chemokine receptor CXCR4 has been shown to signal on mTOR pathway in gastric and renal cancer. CXCR4 interacts with the chemokine CXCL12 to exert proliferative and chemotactic effects.

Aim(s): To evaluate expression and prognostic role of the CXCR4-CXCL12-CXCR7 axis and its relationship with the mTor pathway in NET.

Materials and methods: 61 human NETs were included: 40 GEP-NETs and 21 MTC were analyzed for expression of CXCR4/CXCL12/CXCR7 and mTOR pathways by immunohistochemistry (IHC). CXCR4/CXCR7 was evaluated on NCIH727 and BON cell lines by qRT-PCR and Western-Blot. The effect of mTOR and CXCR4 inhibitors (RAD001, AMD3100) was assessed by MTT assay.

Conference: 12th Annual ENETSConcerence (2015)

Presenting Author: Sciammarella C

Authors: Sciammarella C, Circelli L, Guadagno E, Tafuto S, Del Basso De Caro M,

Keywords: chemokine, mtor, nets, clinical outcome.,