Genome-Wide DNA Methylation Profiling of Pancreatic Neuroendocrine Tumors

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Introduction: Frequent inactivation of epigenetic regulators ATRX and DAXX in pancreatic neuroendocrine tumors (PNETs) suggests an important role for epigenetic alterations in tumorigenesis. Few studies have been published regarding differential methylation patterns in PNETs and a thorough analysis of these alterations is currently lacking.

Aim(s): The aim of this study was to investigate genome-wide changes in DNA methylation associated with PNETs and subsequently evaluate their biomarker potential.

Materials and methods: Fresh-frozen tumor and paired normal tissue of 10 G1/2 PNET patients was bisulfite converted and subjected to genome-wide methylation analysis using Illumina’s Infinium MethylationEPIC BeadChips that target more than 850.000 CpGs. After quality control and normalization, β-values for each CpG were calculated. Based on these β-values, differentially methylated probes (DMPs), regions (DMRs) and blocks (DMBs) were identified using the ChAMP Bioconductor Package. Next, network analysis and pathway analysis were performed to identify altered networks and pathways associated with PNETs.

Conference: 15th Annual ENETSConcerence (2018)

Presenting Author:

Authors: Boons G, Beyens M, Schepers A, Vandamme T, Roeyen G,

Keywords: pancreatic neuroendocrine tumor, DNA methylation,