Heterogeneity and potential therapeutic targets of rectal neuroendocrine tumors by single cell RNA-sequencing combined with spatial omics

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Introduction: The incidence of rectal neuroendocrine tumors (rNETs) has increased rapidly in the past 30 years. However, the molecular mechanism of its occurrence and development is still unclear, and the basic research is relatively backward.

Aim(s): To explore the heterogeneity and tumor microenvironment of rNETs and find potential molecular targets.

Materials and methods: Nine well-differentiated rNETs tissues (5 cases in metastatic group and 4 cases in non-metastatic group) and one liver metastatic tissue were used for single cell RNA sequencing. Four rNETs tissues (2 cases in metastatic group and 2 cases in non-metastatic group) were analysed by spatial transcriptome and metabolomics analysis. Serum, paraffin tissues of patients and primary rNETs cells were used to verify the biological function of target genes with multiple immunohistochemistry, transwell, etc.

Conference:

Presenting Author:

Authors: Bai J, Tang Q,

Keywords: neuroendocrine tumor, single cell RNA sequencing, spatial transcriptome, heterogeneity, tumor microenvironment, phospholipase d3, metastasis,