Integrated Genome-Wide DNA Methylation and mRNA Expression Analysis of Pancreatic NETs Identifies Differential Activation of the Hypoxia Inducible Factor (HIF) Pathway Between Low and Intermediate Grade Tumors


Introduction: This is the first study to integrate DNA methylation and mRNA expression analysis in NETs. It is a powerful approach with which to identify disrupted biological pathways in NET pathogenesis.

Aim(s): To perform an integrated genetic and epigenetic analysis of sporadic pancreatic NET liver metastases to identify biological pathways and targets.

Materials and methods: Ten fresh frozen sporadic pancreatic NET liver tumors (three low grade, three intermediate grade and four high grade) were analysed using the Illumina HumMeth27 beadarray (interrogating 27,500 CpG sites relating to 14,000 genes) and the Affymetrix HumanGene 1.0 ST mRNA expression array (which has 26 probes covering the full coding region of 28,869 genes).

Conference: 8th Annual ENETSConcerence (2011)

Presenting Author:

Authors: Thirlwell C, Schulz L, Eymard M, Luong T, Meyer T,

Keywords: pancreatic neuroendocrine tumor, hypoxia inducible factor, DNA methylation, HIF1-a, IGF1,

To read the full abstract, please log into your ENETS Member account.