Mesenteric fibrosis in SI-NEN is characterized by strong dysregulation of CXCL9, IL6, COMP and COL6A6 genes

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Introduction: Small intestine neuroendocrine neoplasms (SI-NENs) are characterized by the development of desmoplastic mesenteric fibrosis (MF) in up to 50 % of cases. This can cause intestinal obstruction and ischemia, which is a significant clinical problem.

Aim(s): The present study aimed to uncover the molecular pathomechanisms underlying this desmoplastic fiboris.

Materials and methods: 24 SI-NEN patients were selected—13 showing strong MF and 11 who do not. Specimens were evaluated for fibrotic areas within mesenteric lymph node metastases and mesenteric stroma. From these areas of interest, RNA was extracted after micro-dissection and gene expression was evaluated using the HTG EdgeSeq system and Illumina next-generation sequencing. Findings were normalized using DESeq2’s median of ratios method and statistical significance is represented by P < 0.05.

Conference:

Presenting Author:

Authors: Graf S, Keber C, Hattesohl A, Denkert C, Di Fazio P,

Keywords: si-nen, si-net, comp, col6a6, cxcl9, il6, mesenteric fibrosis, desmoplasia, desmoplastic reaction, tumor microenvironment, tme, tumor stroma interaction, next generation sequencing, ngs, htg edgeseq, gene expression analysis, rna,