mTOR Inhibitors Hamper Cell Viability in Selected Human Medullary Thyroid Carcinoma Primary Cultures


Introduction: It has been demonstrated that mTOR inhibitors have potent anti-proliferative effects in a human Medullary Thyroid Carcinoma (MTC) cell line. We here explore the possible role of mTOR inhibitors, Everolimus and BEZ235 (which also inhibits the PI3K pathway) on the effects of Insulin-like Growth Factor-1 (IGF-1) in human MTC primary cultures.

Aim(s): To this purpose, 20 MTCs were dispersed in primary cultures, treated without or with 1uM Everolimus, 10nM-1uM BEZ235, and/or 50nM IGF-I

Materials and methods: Cell viability and apoptosis were evaluated after 48 h. We observed the phosphorylation of p70s6k by ELISA.

Conference: 9th Annual ENETSConcerence (2012)

Presenting Author:

Authors: Minoia m, Gentilin E, Rossi M, Tagliati F, Degli Uberti E,

Keywords: mTOR inhibitors, IGF-1, MTC,

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