Plasma-derived small extracellular vesicles reveal pancreatic neuroendocrine tumors

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Introduction: The incidence of pancreatic neuroendocrine tumors (PNETs) has increased over the past 30 years worldwide. The survival rate is low due to the diagnosis being done at a higher stage and unavailability of specific and sensitive diagnostic markers. Hence, better and efficient early diagnostic biomarkers are required at the earliest. As the role of small extracellular vesicles (sEV) in tumor growth and cancer metastasis is increasing exponentially due to its capability to cause metastasis and proliferation. For the first time, this study establishes the relationship between sEV concentration and PNET grades as well as the presence of BIRC2/cIAP as sEV cargo derived from plasma.

Aim(s): To study the relationship between the sEV concentration and the grades of PNET.

Materials and methods: sEV were isolated from the clarified plasma samples and morphologically characterized using transmission electron microscopy. Further, the expression of sEV was confirmed by sEV markers CD63 & TSG101 and calnexin (negative control). The sEV quantification were performed using nanoparticle tracking analysis (NTA). Later the presence of BIRC2 was analysed as an sEV cargo derived from plasma through western blot and also through immunohistochemistry of PNET tissue and healthy controls (HCs).

Conference:

Presenting Author: Gorai P

Authors: Gorai P, Rastogi S, Soni P, Singh S, Agarwal S,

Keywords: pancreatic neuroendocrine tumor, small extracellular vesicles, BIRC2/cIAP, early diagnostic biomarker,