Rb and p53 Status Determination by Immunochemistry Is Complementary in Routine Practice to Genetic Testing of Neuroendocrine Neoplasms
Introduction: Rb and p53 status may help distinguishing well from poorly differentiated neuroendocrine neoplasms (NEN) and may predict response to chemotherapy. Studies correlating Rb/p53 genetic and immunohistochemistry were performed on high-quality frozen samples with high throughput techniques efficient for copy number determination. This situation differs from routine practice in which small deletions may be hard to detect on formalin-fixed paraffin-embedded (FFPE) samples with a gene panel approach.
Aim(s): Assess the correlation between genetic and immunohistochemistry findings for Rb and p53 in aggressive pancreatic and pulmonary NEN.
Materials and methods: Rb, p16 and p53 protein status were determined by immunohistochemistry (H-score 0-300) in a series of 41 and 71 G3 pancreatic and pulmonary NEN respectively. Genetic testing was performed on 27 tumors with the Oncomine TML (Ion torrent) NGS panel.
Conference: 17th Annual ENETSConcerence (2020)
Presenting Author: Cros J
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