Rb and p53 Status Determination by Immunochemistry Is Complementary in Routine Practice to Genetic Testing of Neuroendocrine Neoplasms


Introduction: Rb and p53 status may help distinguishing well from poorly differentiated neuroendocrine neoplasms (NEN) and may predict response to chemotherapy. Studies correlating Rb/p53 genetic and immunohistochemistry were performed on high-quality frozen samples with high throughput techniques efficient for copy number determination. This situation differs from routine practice in which small deletions may be hard to detect on formalin-fixed paraffin-embedded (FFPE) samples with a gene panel approach.

Aim(s): Assess the correlation between genetic and immunohistochemistry findings for Rb and p53 in aggressive pancreatic and pulmonary NEN.

Materials and methods: Rb, p16 and p53 protein status were determined by immunohistochemistry (H-score 0-300) in a series of 41 and 71 G3 pancreatic and pulmonary NEN respectively. Genetic testing was performed on 27 tumors with the Oncomine TML (Ion torrent) NGS panel.

Conference: 17th Annual ENETSConcerence (2020)

Presenting Author: Cros J

Authors: Chen R, Lacroix L, De Rycke O, Lacombe C, Cazes A,

Keywords: rb, p53, aggressive neuroendocrine tumors, biomarker,

To read the full abstract, please log into your ENETS Member account.