Spatial and temporal intratumour heterogeneity of pancreatic neuroendocrine tumours (PanNET)

#4544

Introduction: Data derived from the study of intertumour heterogeneity have led to propose that PanNET evolution may start either from beta cells with good prognosis, or from alpha cells with sequential alterations of MDA (MENIN-DAXX/ATRX) leading to more aggressive lesions.

Aim(s): We aimed to explore PanNET temporal and spatial intratumour heterogeneity, and the biological mechanisms underlying their progression.

Materials and methods: Three cohorts were used: 1) 286 sporadic PanNET and serial tumour samples over time from 109 patients, 2) 211 resected PanNET, (Ki-67 index and MDA status were assessed for both), 3) 12 highly heterogeneous PanNET (intratumour delta Ki-67 >15%) with multiregional sampling for DNAseq (Panel 571 genes) and RNAseq.

Conference:

Presenting Author: Awan Z

Authors: Awan Z, Bourdeleau P, Epée J, Ps H, Tihy M,

Keywords: Pancreatic neuroendocrine tumour, Heterogeneity, Tumour microenvironment,