Spatial transcriptomics identifies Wnt signalling in multifocal ileal neuroendocrine tumours

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Introduction: Ileal neuroendocrine tumours (i-NETs) often present with multiple primary tumours (>30-40%). Recent studies showed no shared somatic mutations in multiple primary tumours, highlighting the need to examine the tumour microenvironment in tumorigenesis. The Wnt signalling pathway is crucial for organ stem cell regulation. While the Wnt/β-catenin pathway influences prostate and pancreatic NET/Cs, its role in i-NETs remains unclear.

Aim(s): This study comprehensively evaluates expression patterns of Wnt-related genes in multifocal i-NET samples with spatial resolution to identify Wnt signalling involved in i-NET development and progression.

Materials and methods: FFPE blocks of surgically resected specimens from 4 patients with multifocal i-NETs were used. Tissue microarrays were constructed from 72 cores (18 one-mm cores per patient). Spatial gene expression libraries were constructed using Visium v1 (10x Genomics).

Conference:

Presenting Author:

Authors: Yogo A, Akanuma N, Kim G, Thirlwell C, Mäkinen N,

Keywords: Wnt Signalling, Spatial Transcriptomics,