Splicing machinery dysregulation in low grade pulmonary carcinoids

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Introduction: Dysregulation of alternative splicing has emerged as a hallmark for cancer. Recently, our group has shown that splicing machinery expression profile is altered in several cancers, including neuroendocrine tumors. In this study, we explored how this machinery is dysregulated in pulmonary carcinoids.

Aim(s): To dissect the dysregulation of the splicing machinery in pulmonary carcinoids and explore its potential association with clinical parameters.

Materials and methods: A qPCR array was used to measure the expression of 45 components of the splicing machinery in tumor and non-tumor adjacent tissue samples of a cohort of 33 pulmonary NETs. Results were validated using an external cohort of 51 patients. Further analyses were made with immunohistochemistry. Potential associations between expression levels of all genes measured and clinical parameters of patients were assessed. In vitro assays are currently being performed to test the functional role of selected splicing machinery components in carcinoid cell lines H727 and UMC-11.

Conference: 18th Annual ENETS Concerence (2021)

Presenting Author: Blázquez-Encinas R

Authors: Blázquez-Encinas R, Caro M, García-Vioque V, Pedraza-Arévalo S, Alors-Pérez E,

Keywords: neuroendocrine, carcinoid, lung, splicing,